Which analyte is affected by icterus?

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Multiple Choice

Which analyte is affected by icterus?

Icterus introduces bilirubin, which colors the sample yellow and absorbs light in the visible range, especially around 450 nm. When a test relies on colorimetric detection, this bilirubin absorption can bias the signal and skew results. Estradiol (E2) is often measured by colorimetric immunoassays, making it more susceptible to this kind of interference. In contrast, assays for ACTH, TSH, and insulin frequently use luminescent or chemiluminescent readouts (or methods with steps that separate color interference), so they are less affected by icterus. So, estradiol is the analyte most impacted by icterus among these options.

Icterus introduces bilirubin, which colors the sample yellow and absorbs light in the visible range, especially around 450 nm. When a test relies on colorimetric detection, this bilirubin absorption can bias the signal and skew results. Estradiol (E2) is often measured by colorimetric immunoassays, making it more susceptible to this kind of interference. In contrast, assays for ACTH, TSH, and insulin frequently use luminescent or chemiluminescent readouts (or methods with steps that separate color interference), so they are less affected by icterus. So, estradiol is the analyte most impacted by icterus among these options.

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