In serum protein electrophoresis, what is the correct top-to-bottom order of bands?

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Multiple Choice

In serum protein electrophoresis, what is the correct top-to-bottom order of bands?

In serum protein electrophoresis, proteins separate based on their net charge at the buffer pH, with the most negatively charged and smallest molecules moving fastest toward the anode. Albumin has the greatest negative charge and smallest size among the major serum proteins, so it travels the farthest and appears as the first (top) band. The remaining globulins follow in order of decreasing mobility: alpha-1, alpha-2, beta, and finally gamma, whose immunoglobulins migrate the slowest and form the band closest to the opposite end. Therefore, the typical top-to-bottom arrangement is albumin, alpha-1, alpha-2, beta, gamma.

In serum protein electrophoresis, proteins separate based on their net charge at the buffer pH, with the most negatively charged and smallest molecules moving fastest toward the anode. Albumin has the greatest negative charge and smallest size among the major serum proteins, so it travels the farthest and appears as the first (top) band. The remaining globulins follow in order of decreasing mobility: alpha-1, alpha-2, beta, and finally gamma, whose immunoglobulins migrate the slowest and form the band closest to the opposite end. Therefore, the typical top-to-bottom arrangement is albumin, alpha-1, alpha-2, beta, gamma.

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